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t myod dprip  (Addgene inc)


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    Structured Review

    Addgene inc t myod dprip
    T Myod Dprip, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/t+myod+dprip/LV-TRE-WT+mouse+MyoD-T2A-dsRedExpress2+(Plasmid+%2360624)/pmc04461500-106-26-27
    Average 85 stars, based on 2 article reviews
    t myod dprip - by Bioz Stars, 2026-09
    85/100 stars

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    Related Articles

    Sequencing:

    Article Title: Single-Molecule Analysis of Myocyte Differentiation Reveals Bimodal Lineage Commitment
    Article Snippet: An NsiI site and DNA sequence encoding the T2A ribosomal skipping peptide were added upstream of the coding sequence for DsRed-Express2 (Addgene plasmid 21770) by PCR, and the resultant amplicon was inserted into TPriP using the MluI and NheI restriction sites to produce TDPriP. .. The MyoD sequence from EMSV-MyoD 27 was amplified without the stop codon by PCR and inserted into TDPriP at the MluI and NsiI sites to produce T-MyoD-DPriP (Addgene plasmid #60624). .. In order to generate a longer mRNA sequence for the purposes of increased smFISH labeling, a stop codon was placed at the beginning of a LacZ coding sequence from pFRT/lacZeo (Invitrogen V6015) via PCR, which was inserted in place of the DsRed-Express2 sequence between the NsiI and NheI restriction sites to produce T-MyoD-ZPriP (Addgene plasmid #61441).

    Amplification:

    Article Title: Single-Molecule Analysis of Myocyte Differentiation Reveals Bimodal Lineage Commitment
    Article Snippet: An NsiI site and DNA sequence encoding the T2A ribosomal skipping peptide were added upstream of the coding sequence for DsRed-Express2 (Addgene plasmid 21770) by PCR, and the resultant amplicon was inserted into TPriP using the MluI and NheI restriction sites to produce TDPriP. .. The MyoD sequence from EMSV-MyoD 27 was amplified without the stop codon by PCR and inserted into TDPriP at the MluI and NsiI sites to produce T-MyoD-DPriP (Addgene plasmid #60624). .. In order to generate a longer mRNA sequence for the purposes of increased smFISH labeling, a stop codon was placed at the beginning of a LacZ coding sequence from pFRT/lacZeo (Invitrogen V6015) via PCR, which was inserted in place of the DsRed-Express2 sequence between the NsiI and NheI restriction sites to produce T-MyoD-ZPriP (Addgene plasmid #61441).

    Polymerase Chain Reaction:

    Article Title: Single-Molecule Analysis of Myocyte Differentiation Reveals Bimodal Lineage Commitment
    Article Snippet: An NsiI site and DNA sequence encoding the T2A ribosomal skipping peptide were added upstream of the coding sequence for DsRed-Express2 (Addgene plasmid 21770) by PCR, and the resultant amplicon was inserted into TPriP using the MluI and NheI restriction sites to produce TDPriP. .. The MyoD sequence from EMSV-MyoD 27 was amplified without the stop codon by PCR and inserted into TDPriP at the MluI and NsiI sites to produce T-MyoD-DPriP (Addgene plasmid #60624). .. In order to generate a longer mRNA sequence for the purposes of increased smFISH labeling, a stop codon was placed at the beginning of a LacZ coding sequence from pFRT/lacZeo (Invitrogen V6015) via PCR, which was inserted in place of the DsRed-Express2 sequence between the NsiI and NheI restriction sites to produce T-MyoD-ZPriP (Addgene plasmid #61441).

    Plasmid Preparation:

    Article Title: Single-Molecule Analysis of Myocyte Differentiation Reveals Bimodal Lineage Commitment
    Article Snippet: An NsiI site and DNA sequence encoding the T2A ribosomal skipping peptide were added upstream of the coding sequence for DsRed-Express2 (Addgene plasmid 21770) by PCR, and the resultant amplicon was inserted into TPriP using the MluI and NheI restriction sites to produce TDPriP. .. The MyoD sequence from EMSV-MyoD 27 was amplified without the stop codon by PCR and inserted into TDPriP at the MluI and NsiI sites to produce T-MyoD-DPriP (Addgene plasmid #60624). .. In order to generate a longer mRNA sequence for the purposes of increased smFISH labeling, a stop codon was placed at the beginning of a LacZ coding sequence from pFRT/lacZeo (Invitrogen V6015) via PCR, which was inserted in place of the DsRed-Express2 sequence between the NsiI and NheI restriction sites to produce T-MyoD-ZPriP (Addgene plasmid #61441).



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    t myod dprip  (Addgene inc)
    85
    Addgene inc t myod dprip
    T Myod Dprip, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/t+myod+dprip/LV-TRE-WT+mouse+MyoD-T2A-dsRedExpress2+(Plasmid+%2360624)/pmc04461500-106-26-27
    Average 85 stars, based on 1 article reviews
    t myod dprip - by Bioz Stars, 2026-09
    85/100 stars
    		  Buy from Supplier

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